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Image Search Results
Journal: Frontiers in Oncology
Article Title: Identification of UL69 Gene and Protein in Cytomegalovirus-Transformed Human Mammary Epithelial Cells
doi: 10.3389/fonc.2021.627866
Figure Lengend Snippet: Detection of UL69 transcript and pUL69 protein in CTH cells. (A) RT-PCR assay to detect the presence of UL69 transcript in RNA extracts of CTH cells in the presence (+) and absence (-) of reverse transcriptase (RT). Infected HMECs were used as a positive control and no template control (NTC) as a negative control. (B) Detection of pUL69 protein in CTH cells by western blot. (C) Two residue point mutations present in pUL69 in CTH cells when compared to the original pUL69 from HCMV-DB isolate.
Article Snippet: RNA was extracted from CTH cells using
Techniques: Reverse Transcription Polymerase Chain Reaction, Reverse Transcription, Infection, Positive Control, Control, Negative Control, Western Blot, Residue